MET-2 binds and colocalizes in nuclear foci with LIN-65 and ARLE-14. (A) Scheme of structured and disordered domains of MET-2 and LIN-65 proteins. (B) FLAG-LC-MS/MS of MET-2 endogenously tagged with FLAG::mCherry. N = 3 biological replicates. Statistical analysis was done with Perseus (red lines indicate an FDR ≤ 0.1 and curve bend [SO] = 2). (C) FLAG-LC-MS/MS of LIN-65 endogenously tagged with FLAG::GFP. N = 3 biological replicates. Statistical analysis was done as in A. (D) Live-cell images of 2-, 4-, 20-, and ∼100-cell-stage embryos expressing LIN-65::FLAG::GFP or GFP::3xFLAG::ARLE-14. Bar = 5 µm. (E) Localization of MET-2 and LIN-65 in embryos with endogenous met-2 tagged with flag::mcherry (gw1419; red) and endogenous lin-65 tagged with flag::GFP (gw1578; green). Bar = 5 µm. (F) Localization of MET-2 and ARLE-14 in embryos with endogenous met-2 tagged with flag::mcherry(gw1419; red) and endogenous arle-14 tagged with 3xflag::GFP (gw1623; green). Bar = 5 µm.