Figure 5.
Figure 5. Overexpression of DN Vinculin leads to significantly fewer nfatc1:GAL4-expressing ECs in the AVC. (A–F) 3D images of heart valves in WT (A–C) and DN Vinculin–overexpressing (D–F) animals. Shown are sagittal views (left) and atrium-to-ventricle views (right). (G) Number of AV ECs, marked by nfatc1:GAL4; UAS:EGFP and kdrl:nls-mCherry expression, in WT and DN Vinculin–overexpressing animals imaged over a 48-h time window (P = 4.48e−5 at 77 hpf, P = 1.6e−5 at 105 hpf). Error bars represent standard deviation; asterisks indicate statistical significance. (H) Increase in the number of AV ECs between 56 and 77 hpf and 77 and 105 hpf in WT and DN Vinculin–overexpressing animals. WT, n = 12; DN Vinculin, n = 10; P = 2.24e−5. An unpaired Student’s t test was used for G and H. (I and J) 3D images of 100-hpf heart valves. Scale bars: 10 µm.

Overexpression of DN Vinculin leads to significantly fewer nfatc1:GAL4-expressing ECs in the AVC. (A–F) 3D images of heart valves in WT (A–C) and DN Vinculin–overexpressing (D–F) animals. Shown are sagittal views (left) and atrium-to-ventricle views (right). (G) Number of AV ECs, marked by nfatc1:GAL4; UAS:EGFP and kdrl:nls-mCherry expression, in WT and DN Vinculin–overexpressing animals imaged over a 48-h time window (P = 4.48e−5 at 77 hpf, P = 1.6e−5 at 105 hpf). Error bars represent standard deviation; asterisks indicate statistical significance. (H) Increase in the number of AV ECs between 56 and 77 hpf and 77 and 105 hpf in WT and DN Vinculin–overexpressing animals. WT, n = 12; DN Vinculin, n = 10; P = 2.24e−5. An unpaired Student’s t test was used for G and H. (I and J) 3D images of 100-hpf heart valves. Scale bars: 10 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal