Dynein is required for TST of migrating neurons. (A–D) E16 brains were in utero electroporated with control vector or LIC2 G domain and were analyzed by fixed and live imaging 4 d.p.i. Brain slices were stained for the post-mitotic neuronal marker, Tbr1. The CP was equally divided in bins for quantifications purposes. (A) Images of the CP for each condition. (B) Quantification of the proportion of electroporated cells in each bin of the CP. (C) Magnification of the delimited region in bottom right panel in A. Arrowheads mark the elongated migrating process. (D) Time-lapse images for control and LIC2 G domain in migrating neurons. Images are shown at 60-min intervals (hh:mm). Respective representative tracings from multiple migratory neurons for each condition are shown at right (Videos 7 and 8). Data are presented as mean ± SD in B, and unpaired t test was used (**, P < 0.01). Data in B includes at least 3,035 cells from at least six embryos. Bars: 40 µm (A and C); 10 µm (D).