Distinct roles for the LICs in post-mitotic neurons. (A–E) E16 rat embryonic brains were in utero electroporated with shRNAs for LIC1 and/or LIC2, or functional domains of each LIC. Analysis was performed 4 d.p.i. (A and B) Images of the neocortex injected with control vector or shRNAs for LIC1 and/or LIC2 (A) and full-length or G domain versions of LIC1 or LIC2 (B). (C and D) Quantification of the proportion of transfected cells in each layer of the neocortex across conditions. (E) Quantification of the number of multipolar versus uni-/bipolar E20 neurons in the neocortex. (F) Time-lapse images for control, LIC1 G domain, or LIC2 G domain in migrating neurons (Videos 4, 5, and 6). Images are shown at 60-min intervals (hh:mm). Representative tracings are shown at the bottom. Data presented as mean ± SD. Unpaired t test was used in C, D, and E. (**, P < 0.01; ***, P < 0.001). Data in C include at least 2,130 cells from at least six embryos, data in D include at least 1,856 cells from at least three embryos, and data in E include at least 335 cells from at least five embryos. Bars: 100 µm (A and B); 10 µm (F).