Mechanisms contributing to the differential LIC roles in apical INM. (A–D) E16 brains were in utero electroporated with control vector, the full-length, or G domain of either LIC. Analysis was performed at 4 d.p.i. (A) Fixed images of the VZ from electroporated brains stained for PH3. (B and C) Quantification of the distance between RGP nuclei and the VS across the various conditions. (D) Effect of the various cDNAs expression on RGP mitotic index. (E–H) shRNA for LIC1 was injected into the embryonic brain at E16 together with GFP alone, HA-tagged LIC1 RNAi-resistant, or FLAG-tagged standard LIC2 cDNA and analyzed 4 d.p.i. (E) Fixed images of the VZ from electroporated brains stained for PH3 and epitope tag. (F and G) Quantifications of the distance between RGP nuclei and the VS across the various conditions. (H) Mitotic index for the LIC1 rescue and LIC2 cross-rescue. Although statistically nonsignificant (P = 0.06), LIC2 partially reestablishes mitotic index. (I and J) BicD2 pull-down from embryonic rat brain lysate was evaluated for coimmunoprecipitation with dynein HC, IC, and LIC subunits. (J) Quantification of the signal intensity of LIC1 versus LIC2 in the input lane and the IP lane. Data presented as box-and-whiskers plot in B and F; data shown as mean ± SD in C, D, G, H, and J. Unpaired t test was used in B, C, D, F, G, H, and J. (*, P < 0.05; ***, P < 0.001). Data in B, C, and D include at least 343 RGPs from at least three embryos, and data in F, G, and H include at least 1,574 RGPs from at least six embryos. Data in J include four different pull-downs. Bars, 5 µm (A and E).