Figure 1. Distinct roles for the LICs in... | Rockefeller University Press

Figure 1.

$Figure 1. Distinct roles for the LICs in apical INM. (A and B) Diagrammatic representation of the dynein-dynactin-BicD2 complex (A is modified from Carter et al., 2016). LIC1 versus LIC2 content defines distinct dynein subfractions (Tynan et al., 2000a). (B) LIC functional domains and interactors. (C–G) E16 rat brains were in utero electroporated with control vector or shRNAs for LIC1 and/or LIC2 and subsequently imaged live or fixed 4 d post injection (d.p.i.), at E20. (C) Fixed images of the VZ from electroporated brains stained for the mitotic marker phosphohistone-H3 (PH3). Dashed line represents the VS. (D, E, and I) Quantification of distance between the RGP nuclei and the VS across conditions. (F) Effect of LIC1 and/or LIC2 KD on RGP cell mitotic index. (G) Time-lapse images for control, LIC1 KD, or LIC2 KD in RGPs (Videos 1, 2, and 3). Images are shown with 60-min intervals (hh:mm). (H and I) E16 brains were in utero electroporated with BicD2 on a wild-type or LIC1 KD background or LIC1 KD alone. Analysis was done 4 d.p.i. (H) Representative images from electroporated RGPs in the VZ. Data presented as box and whiskers plot in D; and data shown as mean ± SD in E, F, and I. Unpaired t test was used in D, E, F and I. (*, P < 0.05; ***, P < 0.001). Data in D, E, and F include at least 337 RGPs from at least five embryos, and data in I include at least 165 RGPs from at least four embryos. Bars: 5 µm (C and H); 10 µm (G).$

Distinct roles for the LICs in apical INM. (A and B) Diagrammatic representation of the dynein-dynactin-BicD2 complex (A is modified from Carter et al., 2016). LIC1 versus LIC2 content defines distinct dynein subfractions (Tynan et al., 2000a). (B) LIC functional domains and interactors. (C–G) E16 rat brains were in utero electroporated with control vector or shRNAs for LIC1 and/or LIC2 and subsequently imaged live or fixed 4 d post injection (d.p.i.), at E20. (C) Fixed images of the VZ from electroporated brains stained for the mitotic marker phosphohistone-H3 (PH3). Dashed line represents the VS. (D, E, and I) Quantification of distance between the RGP nuclei and the VS across conditions. (F) Effect of LIC1 and/or LIC2 KD on RGP cell mitotic index. (G) Time-lapse images for control, LIC1 KD, or LIC2 KD in RGPs (Videos 1, 2, and 3). Images are shown with 60-min intervals (hh:mm). (H and I) E16 brains were in utero electroporated with BicD2 on a wild-type or LIC1 KD background or LIC1 KD alone. Analysis was done 4 d.p.i. (H) Representative images from electroporated RGPs in the VZ. Data presented as box and whiskers plot in D; and data shown as mean ± SD in E, F, and I. Unpaired t test was used in D, E, F and I. (*, P < 0.05; ***, P < 0.001). Data in D, E, and F include at least 337 RGPs from at least five embryos, and data in I include at least 165 RGPs from at least four embryos. Bars: 5 µm (C and H); 10 µm (G).