Cell polarity modulates centrosomes separation in AB via PLK-1 and LIN-5. Schematic representation of a KLP-7–depleted two-cell stage embryo consisting of an AB cell on the right with anterior PAR proteins at the cortex (green line) and a P1 cell on the left with the posterior PAR proteins at the cortex (red line). Nuclei are represented by gray circles, centrosomes by dark green spheres, and microtubules by green lines. PLK-1 is enriched in the AB cell (dark blue), resulting in low LIN-5 levels and activity (thin orange line) at the cortex. In contrast, in P1, low PLK-1 concentrations (light blue) allow LIN-5 to localize at the cortex (thick orange line) and exert a cortical pulling force, allowing the proper separation of the centrosomes.