PLK-1 limits LIN-5 cortical localization in AB. (A) Confocal images of fixed early two-cell stage embryos expressing endogenously tagged LIN-5::mNG. LIN-5 is observed at the centrosomes, in the cytoplasm, and at the cell cortex. (B) Cortical LIN-5::mNG intensity profiles of AB and P1 cells in control and plk-1(RNAi) embryos. The line profile represents the mean intensities of 28 control and 29 plk-1(RNAi) embryos. In the line profile, the asterisks show the statistical difference compared with WT AB cells. (C) Quantifications of LIN-5::mNG intensities integrated over a cortical stripe after a Z projection in control and plk-1(RNAi) AB and P1 cells (see Materials and methods for details). Scale bar, 5 µm. The statistical test used is indicated in Table S1. *, P ≤ 0.05; ***, P ≤ 0.001; ****, P ≤ 0.001.