A mutation in LIN-5 abolishes the difference of behavior of centrosomes in AB and P1 cells. (A) Still images of AB and P1 cells taken from video recordings of embryos of the indicated genotypes. The FM102 strain containing the lin-5(ev571ts) mutation and its parental strain (AZ235) were used in this experiment. (B) Quantification of the centrosome separation ratios of the indicated genotypes. (C) Still images of AB and P1 cells from video recordings of JDU316 (containing the klp-7(tm2143) deletion) and its parental strain, JCC456, with the indicated genotypes. (D) Quantification of the centrosome separation ratios of the indicated genotypes. Scale bars, 5 µm. The number of cells/embryos analyzed is indicated at the bottom of the graph and the statistical test used is indicated in Table S1. *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.001.