Depletion of MEX-5/6 in the klp-7(tm2143) mutant results in centrosome separation defects in P1. (A) Images of early fixed two-cell stage control and mex-5/6(RNAi) embryos expressing endogenously tagged PLK-1::GFP. (B) Absolute intensity quantification of PLK-1::GFP in control and mex-5/6(RNAi) AB and P1 cells. Errors bars indicate SEM. (C) Still images from time-lapse recording of AB and P1 cells from embryos treated with the indicated depletions. JDU316 (containing the klp-7(tm2143) deletion) and its parental strain, JCC483, were used in this experiment. (D) Quantification of the centrosome separation ratios of the indicated genotypes. Schematic models at the bottom indicate the polarity status (anterior = green; posterior = red) and the relative PLK-1 levels (high levels = dark blue; intermediate levels = blue; low levels = light blue). Scale bars, 5 µm. The number of cells/embryos analyzed is indicated at the bottom of the graph and the statistical test used is indicated in Table S1. *, P ≤ 0.05; ***, P ≤ 0.001; ****, P ≤ 0.001.