The centrosome separation defect of KLP-7–depleted embryos depends on cell polarity. (A) Schematic representation of the experiment: WT two-cell embryo with localization of anterior PAR proteins (in green) around AB and posterior PAR proteins (in red) around P1 on the left. Schematic representation of a par-2(RNAi) embryo on the top right and of a par-3(RNAi) embryo on the bottom right. The gray spheres represent the nuclei, and the black full circles are the centrosomes. (B) Still images of AB and P1 cells before NEBD taken from recordings of embryos treated with the indicated RNAi. JDU316 (containing the klp-7(tm2143) deletion) and its parental strain, JCC483, were used in this experiment. (C) Quantification of the centrosome separation ratios of the indicated genotypes. Model two-cell embryos indicate the polarity status (anterior in green; posterior in red) of the respective cell. Scale bar, 5 µm. The number of cells/embryos analyzed is indicated at the bottom of the graph and the statistical test used is indicated in Table S1. *, P ≤ 0.05; ***, P ≤ 0.001.