Centrosome separation is defective in KLP-7 depleted embryos. (A) Still images of the AB (left) and P1 cell (right) taken from time-lapse videos of a YFP::α-tubulin expressing strain (TH65) with the indicated depletions. For all the subsequent figures of centrosome position in AB and P1 throughout the paper, the images represent the maximum intensities of Z projection at the time point just before NEBD (15 s before NEBD), the dashed yellow line highlights the nucleus, centrosomes are highlighted with a blue circle (or dashed blue circle when not in focus), and the cell periphery is delimited by the dashed gray line. (B) Quantification of centrosome separation ratios (CTR-CTR) as illustrated in Fig. S1. In this and all subsequent figures, ratios are plotted as box plots (dark color in AB, light color in P1). (C) Still images of the AB (left) and P1 cell (right) taken from time-lapse videos of a GFP::β-tubulin expressing strain (JDU316, containing the klp-7(tm2143) deletion, and its parental strain, JCC483). (D) Quantification of centrosome separation ratios in the indicated genotypes. Scale bars, 5 µm. The number of cells/embryos analyzed is indicated at the bottom of the graph and the statistical test used is indicated in Table S1. **, P ≤ 0.01; ***, P ≤ 0.001.