Figure 8.
![Figure 8. Lipidated probes move through the connecting cilium via impeded diffusion. (A and C) Time course images of PAGFP or Far0-PAGFP flux into the IS. Vertical lines indicate the OS–IS junction. At time zero, PAGFP was activated by two photon scanning over 70–80% of the OS. ROIs were drawn to find cOS (ROI1), cIS (ROI2); and MIS (ROI3; Eq. 1). Scale bars, 10 µm. (B and D) Flux normalized to OS–IS concentration gradient (grad [OS-IS]). Line is linear regression. DCC was estimated according to Eq. 1. (E) Average DCCs; error bars: SEM. Asterisk indicates DCC of PAGFP was significantly higher than those of the lipidated probes at P = 0.05, as determined by two-tailed t test.](https://cdn.rupress.org/rup/content_public/journal/jcb/218/11/10.1083_jcb.201906024/9/jcb_201906024_fig8.jpeg?Expires=1771774887&Signature=viavhMiRNFemYFPPIlhKy5r6xg5bUx1MtSorec8PphXA~imYpb3LDP7S8Fb0-nNl~n0OGla~Bi~g9Irq4mOjv-3nXN0q4Ot8igiuHgYH6kC4L-bZSNIwgo1wvqtEXw8W5i1U06le3a1emuZ9VrkNqucK7LN-~hcKGsy7i6a1y2xQQarFgORiEdW989mqdUnVZLQU6Dv2bsZCgb1mTggviNRjtC5Bc32lBmxHHnrR2KOwkeFLmQwskl4~KKBYuPmwY9ovlVxS38y5uY8pFQaGE13zFiGoeh8u-~NTpUZqF4BBWrFSZsQz1xy6tvxqMKA73HuBbctj9rlatbgMKpqeKA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Lipidated probes move through the connecting cilium via impeded diffusion. (A and C) Time course images of PAGFP or Far0-PAGFP flux into the IS. Vertical lines indicate the OS–IS junction. At time zero, PAGFP was activated by two photon scanning over 70–80% of the OS. ROIs were drawn to find cOS (ROI1), cIS (ROI2); and MIS (ROI3; Eq. 1). Scale bars, 10 µm. (B and D) Flux normalized to OS–IS concentration gradient (grad [OS-IS]). Line is linear regression. DCC was estimated according to Eq. 1. (E) Average DCCs; error bars: SEM. Asterisk indicates DCC of PAGFP was significantly higher than those of the lipidated probes at P = 0.05, as determined by two-tailed t test.
