Myristoylated probes are enriched in the OS regardless of linker charge. (A) Upper panels: Representative confocal images of rods expressing Myr probes. Lower panels: Compartment distribution profiles. (B) Confocal image and compartment distribution profile of Tαnt16. (A and B) Scale bars, 10 µm. (C) OSEIs of myristoylated probes. Box-whisker plots as described in Fig. 2. Significance table shows that all probes had significantly higher OSEIs than NL0. (D) SynEIs show that Myr+8 is the only presynapse enriched probe. Significance tables: P ≤ 0.05; NS, not significant. n for each construct: Myr+8, 27; Myr0, 28; Myr-8, 15; and Tαnt16, 18.