Figure 5.
Figure 5. Prenylated PMP transport to OS does not require association with LBC protein, PrBPδ. (A–C) GFP trap of prenylated probes fail to pull down PrBPδ. Western blots probed with anti PrBPδ antibody. (D and E) Upper panels: Representative confocal images of GRK1 and Tγ probes. Scale bars, 10 µm. Lower panels: Normalized compartment distributions. (F) OSEIs showing higher OS enrichment of GRK1. Significance table: P ≤ 0.05; NS, not significant. (G and H) GFP trap of GRK1 and Tγ probes show that GRK1ct18 associates with PrBPδ while Tγct15 does not. Box-whisker plots as described in Fig. 2. n for each construct: GRK1ct18, 30; Tγct19, 17; Far0, 42; and NL0, 29. (A–C, G, and H) MW, molecular weight markers; I, input; FT, flow through; and E, eluate.

Prenylated PMP transport to OS does not require association with LBC protein, PrBPδ. (A–C) GFP trap of prenylated probes fail to pull down PrBPδ. Western blots probed with anti PrBPδ antibody. (D and E) Upper panels: Representative confocal images of GRK1 and Tγ probes. Scale bars, 10 µm. Lower panels: Normalized compartment distributions. (F) OSEIs showing higher OS enrichment of GRK1. Significance table: P ≤ 0.05; NS, not significant. (G and H) GFP trap of GRK1 and Tγ probes show that GRK1ct18 associates with PrBPδ while Tγct15 does not. Box-whisker plots as described in Fig. 2. n for each construct: GRK1ct18, 30; Tγct19, 17; Far0, 42; and NL0, 29. (A–C, G, and H) MW, molecular weight markers; I, input; FT, flow through; and E, eluate.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal