Distribution of prenylated probes dependds on linker charge. (A–C) Upper panels, representative confocal images. Scale bars, 10 µm. Lower panels, average compartment fluorescence normalized to total cell fluorescence. (D) OSEI, defined as the ratio of average OS fluorescence to average myoid fluorescence, F_OS/F_M. (E) SynEI, defined as ratio of average synaptic fluorescence to average myoid fluorescence, F_syn/F_M. (D and E) Lower panels are significance tables. *, P = 0.05; NS, not significant. Box fills as in Fig. 2 C key. Box-whisker plots as described in Fig. 2. n for each construct: NL+8, 14; NL0, 13; NL-8, 17; GG+8, 14; GG0, 20; GG-8, 22; Far+8, 13; Far+4, 21; Far0, 42; and Far-8, 16.