Figure 7.
Figure 7. The nudAR1602E mutation causes GFP-dynein to accumulate at the septa in gpdA-ΔC-hookA-S cells regardless of NudF/LIS1 function. (A) GFP-dynein with the nudAR1602E mutation accumulates at the septa in gpdA-ΔC-hookA-S cells with or without the nudF6 mutation. Hyphal tips are indicated by arrowheads and septa by arrows. (B) A quantitative analysis on dynein comet intensity in nudF6, gpdA-ΔC-hookA-S (n = 58), nudF6, nudAR1602E, gpdA-ΔC-hookA-S (n = 47), and nudAR1602E, gpdA-ΔC-hookA-S (n = 30) strains. The average value for the nudF6, gpdA-ΔC-hookA-S strain is set as 1. Scatterplots with mean and SD values were generated by Prism 8. ****, P < 0.0001; **, P < 0.01 (Kruskal-Wallis ANOVA test with Dunn’s multiple comparisons test, unpaired).

The nudAR1602E mutation causes GFP-dynein to accumulate at the septa in gpdA-ΔC-hookA-S cells regardless of NudF/LIS1 function. (A) GFP-dynein with the nudAR1602E mutation accumulates at the septa in gpdA-ΔC-hookA-S cells with or without the nudF6 mutation. Hyphal tips are indicated by arrowheads and septa by arrows. (B) A quantitative analysis on dynein comet intensity in nudF6, gpdA-ΔC-hookA-S (n = 58), nudF6, nudAR1602E, gpdA-ΔC-hookA-S (n = 47), and nudAR1602E, gpdA-ΔC-hookA-S (n = 30) strains. The average value for the nudF6, gpdA-ΔC-hookA-S strain is set as 1. Scatterplots with mean and SD values were generated by Prism 8. ****, P < 0.0001; **, P < 0.01 (Kruskal-Wallis ANOVA test with Dunn’s multiple comparisons test, unpaired).

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