Dynein along MTs in the ΔkinA mutant relocates to the minus ends upon ΔC-HookA overexpression, and NudF/LIS1 plays an important role in this process. (A) Images of GFP-dynein in wild type, a ΔkinA strain, a ΔkinA, gpdA-ΔC-hookA-S strain, and a nudF6, ΔkinA, gpdA-ΔC-hookA-S strain. Hyphal tips are indicated by arrowheads and septa by brown arrows. Bars, 5 µm. (B) A quantitative analysis of septal GFP-dynein signal intensity in ΔkinA, gpdA-ΔC-hookA-S (n = 74) and nudF6, ΔkinA, gpdA-ΔC-hookA-S (n = 62) strains. The average value for the ΔkinA, gpdA-ΔC-hookA-S strain is set as 1. Scatterplots with mean and SD values were generated by Prism 8. ****, P < 0.0001 (Student’s t test, two-tailed, unpaired). (C) Western blots showing that dynein HC and dynactin p150 are pulled down with ΔC-HookA-GFP in wild type and the alcA-nudF mutant in which the expression of NudF/LIS1 is shut off. The Δp25 mutant was used as a negative control. (D) A quantitative analysis on the ratio of pulled-down dynein HC to ΔC-HookA-GFP (dynein/ΔC-HookA) and that of pulled-down dynactin p150 to ΔC-HookA-GFP (dynactin/ΔC-HookA). The values were generated from western analyses of four independent pull-down experiments (n = 4 for all). The wild-type values are set as 1. Scatterplots with mean and SD values were generated by Prism 8. **, P < 0.01 (Student’s t test, two tailed, unpaired).