NudF/LIS1 proteins do not relocate to MT minus ends upon overexpression of ΔC-HookA. (A) NudF/LIS1-GFP signals accumulate as plus-end comets, but not at the septum, in a gpdA-ΔC-hookA-S strain. Hyphal tips are indicated by arrowheads and septa by arrows. Bars, 5 µm. (B) A quantitative analysis of the NudF/LIS1-GFP comet intensity in wild-type (n = 57) and gpdA-ΔC-hookA-S (n = 55) strains. Two sets of GFP-dynein data (used in Fig. 2 B) were shown for comparison, but only the wild type (n = 61) was included in the statistical analysis. The average wild-type value for GFP-dynein is set as 1. Scatterplots with mean and SD values were generated by Prism 8. ****, P < 0.0001 (Kruskal-Wallis ANOVA test with Dunn’s multiple comparisons test, unpaired). (C) A Western blot showing that the protein level of NudF/LIS1-GFP (arrow) is not changed apparently upon ΔC-HookA overexpression in the gpdA-ΔC-hookA-S strain. A strain without the NudF/LIS1-GFP fusion is used as a negative control for the anti-GFP antibody. Note that a nonspecific band slightly above the 50-kD marker helps to show equal loading in the three lanes.