NudF/LIS1 is required for the relocation of dynein and dynactin from the MT plus ends to the minus ends upon ΔC-HookA overexpression. (A) GFP-dynein in wild type, a gpdA-ΔC-hookA-S strain, and a nudF6, gpdA-ΔC-hookA-S strain. Note that GFP-dynein accumulates as MT plus-end comets, but not at the septum, in the nudF6, gpdA-ΔC-hookA-S hypha. Bright-field images are shown below to indicate hyphal shape and position of the septum. Hyphal tips are indicated by arrowheads and septa by arrows. (B) A quantitative analysis on dynein comet intensity in the nudF6, gpdA-ΔC-HookA-S strain (n = 61) in comparison to those in wild type (n = 61) and the gpdA-ΔC-HookA-S strain (n = 10). The average wild-type value is set as 1. Scatterplots with mean and SD values were generated by Prism 8. ****, P < 0.0001; **, P < 0.01 (Kruskal–Wallis ANOVA test with Dunn’s multiple comparisons test, unpaired). (C) p150-dynactin-GFP in wild type, a gpdA-ΔC-hookA-S strain, and a nudF6, gpdA-ΔC-hookA-S strain. Note that GFP-dynactin accumulates as plus-end comets, but not at the septum, in the nudF6, gpdA-ΔC-hookA-S strain. (D) GFP-dynein in a nudF7, gpdA-ΔC-hookA-S strain and an alcA-nudF, gpdA-ΔC-hookA-S strain. Note that GFP-dynein plus-end comets are present in these cells. Bars, 5 µm.