Dynamin 2 association with inclusion formation in Myo5b KO mice. (A) Immunostaining for Dynamin 2 (red) in the duodenum of neonatal control mice showed that Dynamin 2 was concentrated below the apical brush border as demarcated by DPPIV (green). The endosomal protein endotubin appeared to be localized immediately below Dynamin 2 in enterocytes. (B) In Myo5b KO enterocytes, Dynamin 2 appeared to concentrate with inclusions that were DPPIV and endotubin positive. In inclusions near the apical membrane, Dynamin 2 was evident in regions where detachment from the apical membrane appeared to be occurring. In fully internalized inclusions, Dynamin 2 was still associated with inclusions, frequently in distinct aggregates. Interestingly, two or more discrete Dynamin 2 regions were often observed in association with a single inclusion. n = 6 mice per group. Scale bars = 5 µm.