Effects of far1^H7 and STE2 mutants on GFP-Gβ localization to the DS. (A–D) Representative time-lapse images. MATa cells expressing reporter genes tagged with GFP in situ were mixed with an equal number of MATα cells and imaged from cytokinesis to the indicated time points. Localization of Far1^H7-GFP (A), GFP-Gβ in MATa far1^H7 cells (B), GFP-Gβ in MATa ste2^7XR cells (C), and GFP-Gβ in MATa ste2^7XR/6SA cells (D). (E) Box scatterplots showing initial polarity angle, δ (see Fig. 1 E). Mean δ ± SEM in degrees: Bud = 33.7 ± 0.9; GFP-Gβ in far1^H7 cells = 7.8 ± 0.6; GFP-Gβ in ste2^7XR cells = 33.8 ± 1.2; GFP-Gβ in ste2^7XR/6SA cells = 33.5 ± 1.1. (F) Box plots showing GFP-Gβ pause times at the bud neck in the indicated backgrounds. Mean pause ± SEM in minutes: WT = 8.9 ± 0.5; far1^H7 = 46.0 ± 3.5; ste2^7XR = 9.0 ± 0.5; ste2^7XR/6SA = 8.9 ± 0.4. n ≥ 50 for all strains and measurements; *, P < 0.0001.