Figure 6.
Figure 6. F-actin shell, meshwork, and patches were formed at all clamped pHi values. (A and B) Oocytes stimulated with 1-MA in ASW or in modified ASW to clamp the pHi at ∼7.2, 7.0, or 6.7 were fixed upon GVBD or 5 min after GVBD and then triple-stained with phalloidin for F-actin (gray), anti-α-tubulin antibody for microtubules (green), and DAPI for chromosomes (magenta). Typical images of GV region (max projections and single slices) from two independent experiments are shown in A. Single slices including the actin patches (white boxes with enlarged images) observed 5 min after GVBD are shown in B. Bars in A and B, (main images) 10 µm, (insets) 5 µm.

F-actin shell, meshwork, and patches were formed at all clamped pHi values. (A and B) Oocytes stimulated with 1-MA in ASW or in modified ASW to clamp the pHi at ∼7.2, 7.0, or 6.7 were fixed upon GVBD or 5 min after GVBD and then triple-stained with phalloidin for F-actin (gray), anti-α-tubulin antibody for microtubules (green), and DAPI for chromosomes (magenta). Typical images of GV region (max projections and single slices) from two independent experiments are shown in A. Single slices including the actin patches (white boxes with enlarged images) observed 5 min after GVBD are shown in B. Bars in A and B, (main images) 10 µm, (insets) 5 µm.

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