Tissue distribution of FAMK-1 expression assessed using transcriptional and translational reporters. (A) Schematic of transcriptional reporter used to analyze expression of famk-1 in the L4 larval stage. The schematic on the right highlights the tissues where famk-1 expression was observed. Images below show overlays of bright-field (BF) and nuclear GFP signals; arrows highlight fluorescent nuclei. The most prominent transcription of famk-1 in the L4 larva (and adult) is in the spermatheca. Asterisk marks background autofluorescence. Scale bars, 10 µm. (B) Image of oocytes in an adult highlighting famk-1 expression in the germline. Scale bar, 10 µm. (C) Immunoblot of embryos purified from the indicated strains. Asterisk marks a background band. α-Tubulin serves as a loading control. (D) Comparison of mCherry-fused WT and KDEL FAMK-1 in the spermatheca and the uterus. For images in other tissues, see Fig. S5. Scale bar, 10 µm.