Hindbrain and retinal nuclei are deformable and experience different forces during apical nuclear migration. (A) Immunostaining of Lamin B1 and Lamin A/C in hindbrain and retinal cells (lookup tables indicate minimal and maximal signal values). (B) Dynamics of nuclear deformations in interphase hindbrain and retinal cells, visualized with nuclear envelope marker EGFP-LAP2b (Video 2). (C) 3D segmentation of hindbrain and retinal nuclei in S and G2. (D) Aspect ratio variability in S and G2 hindbrain (HB) and retinal (R) cells. S-phase nuclei display similar variances in nuclear aspect ratio (P = 0.7892, Levene’s test, Table 1). In G2, the variance and the mean aspect ratio increase more strongly for retinal nuclei (P < 0.0001, Levene’s test, and P < 0.0001, Mann-Whitney test, respectively). (E) Cross-correlation analysis of nuclear instantaneous velocity and aspect ratio changes in the retina shows increased average correlation in G2. Error bars: SD. Scale bars: 5 µm.