Gulp1 regulates the EphB2/ephrinB1-mediated cell disengagement response. (A–C) Time-lapse images (A and B) and quantification (C) showing expression of dominant-negative Gulp1ΔC inhibits EphB2/ephrinB1-mediated cell disengagement. HeLa cells expressing EphB2 and either GFP or GFP-Gulp1ΔC were cocultured with donor cells expressing ephrinB1ΔC-mCherry. Maximum projection of deconvolved images is shown. Scale bars, 10 µm. Elapsed time shown as min:s. Dashed lines indicate the distance between two contacting cells. Measured distance between donor and responder cells over time for each condition shown as mean ± SEM n = 20 (GFP-Gulp1ΔC) and 13 (GFP) donor–responder pairs from three experiments. ***, P < 0.001; two-way ANOVA.