Wdr1-deficient neutrophils lose their nuclear lamina. (A–E) Whole-mount immunofluorescence images of neutrophils in sibling versus mutant Tg(Lyz:GFP) embryos at 2.5 dpf immunostained for Lamin B2 (red), with DNA stained by the far-red fluorescent dye RedDot 1 (cyan). Whereas lamin B2 delineated the nucleus in neutrophils of WT siblings (A, arrows), it did not in any late-stage mutant neutrophil (C and D) nor in some at early stage (B; see also Video 7). Aggregates of lamin B2 were often apparent instead (D, arrow). (E) Proportions of mutant (n = 13) versus WT (n = 16) neutrophils in which a full lamin B2–delineated NE was detected. χ² test; *, P < 0.05. Scale bars, 5 µm.