Cyst localizes to the apico-lateral cortex of epithelial cells. (A) Four z-projections of the ventral ectoderm (0.37-µm step size) showing 5.2-µm depth from a live cyst RNAi embryo rescued by GFP::Cyst^R. GFP::Cyst^R is enriched at the apico-lateral cortex immediately below the vitelline membrane (0.0–0.7 µm). Scale bar, 5 µm. (B) Z-projections of junctional region (0.37-µm step size) of the ventral ectoderm or epidermis from live cyst RNAi embryos rescued by GFP::Cyst^R of the indicated stages. Scale bar, 5 µm.