CRAC/CARC/R-W-L motifs in Bet1 are required for the proximity of MT1-MMP and Bet1. (A) Putative CRAC/CARC/R-W-L motifs in Bet1. (B) Bet1 CRAC/CARC/R-W-L motif mutants exhibit reduced PLA signals to MT1-MMP. Bet1 mutants in which consensus residues were replaced with alanine were transfected into MDA-MB-231 cells, which were subjected to PLA reaction with endogenous MT1-MMP as in Fig. 6 E. (C) Quantitation of PLA signals between MT1-MMP and Bet1-GFP as in B. (D) A hypothetical model of the Bet1 function in invasive and noninvasive cancer cells. Scale bar: 10 μm. **, P < 0.01; vs. WT in C.