Figure 2.
Figure 2. Perturbation of Hsc70 inhibits cargo incorporation. (A) Schematic diagram of Auxilin, miniAuxilin, and miniAuxilin3A. PTEN indicates the phosphatase and tensin homology domain and JD represents the J-domain, which contains the HPD-AAA mutations in the 3A mutant. (B) Representative images of membranes expressing TfR-pHluorin and CCPs after incubation with cytosol and either miniAuxilin or miniAuxilin3A. Plots represent the intensity profiles of TfR-pHluorin and CCP. (C and D) Representative images of membranes expressing GluT4-GFP (C) or VAMP7-pHluorin (D) and CCPs after incubation of cytosol or with miniAuxilin3A. (E) Representative images of membranes expressing TfR-pHluorin and CCPs after incubation of cytosol with Hsc70 or Hsc70K71M. Plots represent the intensity profiles of TfR-pHluorin and CCP. Scale bars, 2 µm.

Perturbation of Hsc70 inhibits cargo incorporation. (A) Schematic diagram of Auxilin, miniAuxilin, and miniAuxilin3A. PTEN indicates the phosphatase and tensin homology domain and JD represents the J-domain, which contains the HPD-AAA mutations in the 3A mutant. (B) Representative images of membranes expressing TfR-pHluorin and CCPs after incubation with cytosol and either miniAuxilin or miniAuxilin3A. Plots represent the intensity profiles of TfR-pHluorin and CCP. (C and D) Representative images of membranes expressing GluT4-GFP (C) or VAMP7-pHluorin (D) and CCPs after incubation of cytosol or with miniAuxilin3A. (E) Representative images of membranes expressing TfR-pHluorin and CCPs after incubation of cytosol with Hsc70 or Hsc70K71M. Plots represent the intensity profiles of TfR-pHluorin and CCP. Scale bars, 2 µm.

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