GFP–COL trafficking does not rely on an intact microtubule network. Still images from confocal live-cell imaging of RPE-1 stably expressing GFP–COL (green; GFP–COL–RPE) cotransfected with the trans-Golgi marker ST-Cherry (magenta) derived from Video 6. Acquisition at one frame every 26 s. Cells were incubated in presence of NZ for 60 min before imaging. Time points indicate time in presence of asc/biotin (500 µg·ml⁻¹ and 400 µM, respectively). (A) Large panels show the entire cell. Scale bar, 10 µm. (B) Enlargements highlight a zoomed in area of interest. Small panels show the green channel and magenta channel in gray scale followed by the overlay. Scale bar, 1 µm. (C) Example image of an enlarged overlay with a 5-pixel-wide line drawn through the Golgi followed by corresponding line graphs of selected time points showing the signal intensity (y axis) in arbitrary units for GFP–COL (green) and ST-Cherry (magenta) for the corresponding line. The x axis shows the distance in micrometers. n = 4.