Heterotypic interactions between autophagosomes and lysosomes happen on detyrosinated microtubules. (A) Snapshots from a time-lapse video where an autophagosome (green) moves toward and interacts with an autolysosome (magenta) on detyrosinated microtubules (white) in cells expressing the tandem fluorescent-tagged LC3B plasmid. The autophagosome trajectory is shown in red. Bars, 500 nm. (B–D) Wide-field images showing autophagosomes (white) and autolysosomes (magenta) in starved cells transiently overexpressing tyrosine ligase enzyme TTL (B) or starved cells transiently expressing Vasohibin 1 shRNA (C). Bar, 2 µm. (D) Quantification of the percentage of autophagosomes (AP) and autolysosomes (AL) in control starved cells (Fig. S2 G) and for the conditions corresponding to B and C. The bars indicate the median, and the error bar is the SD analyzed for 10 different cells in each case. The asterisk indicates the statistical significance for a two-tailed two-sample t test (*, P < 0.05).