Detyrosinated microtubules form a small subset of all microtubules. (A–C) Two-color super-resolution images of total α-tubulin (green), acetylated α-tubulin (magenta), and overlay (A); tyrosinated α-tubulin (green), detyrosinated α-tubulin (magenta), and overlay (B); and acetylated α-tubulin (green), detyrosinated α-tubulin (magenta), and overlay (C). (D) Bar plots show the proportion of detyrosinated α-tubulin, acetylated α-tubulin, and double-modified α-tubulin containing both acetylation and detyrosination marks quantified as the percentage of total microtubule (MT) area corresponding to these PTMs. The bars indicate the median, and the error bar is the SD, analyzed for 10 different cells in each case. (E) Pie diagram showing the major subpopulations of modified microtubules that could be identified from the super-resolution images. Bars are 2 µm (left) and 500 nm (Zoom).