Partially decondensed DNA in Arpp19 knockout MEFs is associated with dephosphorylation of histone H3 and of the condensin II subunit CAPD3. (A) GFP and CRE cells were synchronized in mitosis with nocodazole, and after shake-off, chromosome spreads were prepared. Left: Representative phenotypes observed in chromosome spreads. Right: Quantification of cells displaying the indicated phenotypes. Data were from two independent experiments. (B) GFP- or GFP-Cre–transduced MEFs were used for immunofluorescence analysis with anti-CAPD3 antibodies and confocal microscopy (representative images). Bars, 5 µm. Bar magnifications, 2 µm. (C and D) Mitotically enriched GFP- or GFP-Cre–transduced Arpp19^Lox/Lox MEFs were incubated or not with OA, and then lysates were analyzed by Western blotting. Band intensities from three independent experiments were normalized to the loading controls and are represented as the mean ± SD of three replicates.