Figure 4.
Figure 4. Fir1 prevents Cts1 secretion upon septation failure. Inhibition of Cts1 secretion upon septation failure requires Fir1. (A) Localization of Chs2-GFP-AID in the presence or absence of auxin. At 30 min after mitotic release, Chs2-GFP-AID localized to the bud neck (−Auxin) while in the presence of auxin (+Auxin) GFP signal at the bud neck could not be detected. A representative cell is shown. Bar, 2 µm. (B) chs2-AID and chs2-AID fir1Δ cells expressing HA-tagged Cts1 were synchronized in mitosis and treated with DMSO (−Auxin) or 0.5 mM auxin (+Auxin). Cells were grown at 27°C, collected, and processed as in Fig. 2 B. (C) Western blot quantification (as in Fig. 2 C). (D) FIR1 deletion enhances the lethality of chs2-AID. Fivefold serial dilutions of GalL CDC20 HA-Cts1 strains plus the indicated genotype were spotted to YP galactose plates plus or minus auxin and grown at 27°C.

Fir1 prevents Cts1 secretion upon septation failure. Inhibition of Cts1 secretion upon septation failure requires Fir1. (A) Localization of Chs2-GFP-AID in the presence or absence of auxin. At 30 min after mitotic release, Chs2-GFP-AID localized to the bud neck (−Auxin) while in the presence of auxin (+Auxin) GFP signal at the bud neck could not be detected. A representative cell is shown. Bar, 2 µm. (B) chs2-AID and chs2-AID fir1Δ cells expressing HA-tagged Cts1 were synchronized in mitosis and treated with DMSO (−Auxin) or 0.5 mM auxin (+Auxin). Cells were grown at 27°C, collected, and processed as in Fig. 2 B. (C) Western blot quantification (as in Fig. 2 C). (D) FIR1 deletion enhances the lethality of chs2-AID. Fivefold serial dilutions of GalL CDC20 HA-Cts1 strains plus the indicated genotype were spotted to YP galactose plates plus or minus auxin and grown at 27°C.

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