Loss of Hook1 leads to loss of downstream signaling measured by pCREB levels. (A) Representative images of somas after BDNF-Qdots treatment. (B) Quantification of somas with no BDNF-Qdots present. Bar graph shows mean ± SEM; unpaired t test (*, P = 0.0131). n = 3 individual experimental averages, 36–41 cells. (C) Representative images of soma with pCREB staining. (A and C) Bars, 10 µm. (D) Quantification of nuclear pCREB signaling. Scatter plot shows mean ± SEM; one-way ANOVA (*, P = 0.024; ***, P < 0.0002; ns, P = 0.491). Mock, NT: n = 42 somas; mock + BDNF: n = 48 somas; Hk1 KD, NT: n = 51 somas; Hk1 KD + BDNF: n = 55 somas. (E) Normalized pCREB intensity to the NT mock condition per individual experiment. Bar graph shows mean ± SEM; repeated-measures one-way ANOVA (*, P < 0.047; ns, P = 0.99). n = 3 individual experiments.