Figure 5. Hook1 KD reduces flux of BDNF... | Rockefeller University Press

Figure 5.

$Figure 5. Hook1 KD reduces flux of BDNF from distal axon. (A) Schematic of the microfluidic device and experimental setup. (B) Kymographs of BDNF in MOCK, Hk1 KD, and Hk3 KD neurons grown in the microfluidic device. Arrows point to retrograde events. Bars: 10 µm (horizontal); 10 s (vertical). (C) Motility fractioned into retrograde, anterograde, and nonmotile events per neuron. Bar graph shows mean ± SEM; two-way ANOVA (ns, P > 0.113) Mock: n = 35 neurons; Hk1 KD: n = 34 neurons. (D) Quantification of flux of BDNF-Qdots in mid-axons. Scatter plot shows mean ± SEM; one-way ANOVA (***, P < 0.0001; ns, P = 0.9994). Mock: n = 54 neurons; Hk1 KD: n = 36 neurons; Hk3 KD: n = 29 neurons. (E) Number of switches in BDNF-Qdot events. Scatter plot shows mean ± SEM; Mann–Whitney t test (**, P = 0.001). Mock: n = 110 events; Hk1 KD: n = 84 events. (F) Pause duration of retrograde BDNF-Qdot events. Scatter plot shows mean ± SEM, Mann-Whitney t test (**, P = 0.0062). Mock: n = 57 events; Hk1 KD: n = 33 events. (G) Net velocity of retrograde BDNF-Qdot events. Scatter plot shows mean ± SEM, unpaired t test (**, P = 0.0061). Mock: n = 63 events; Hk1 KD: n = 34 events.$

Hook1 KD reduces flux of BDNF from distal axon. (A) Schematic of the microfluidic device and experimental setup. (B) Kymographs of BDNF in MOCK, Hk1 KD, and Hk3 KD neurons grown in the microfluidic device. Arrows point to retrograde events. Bars: 10 µm (horizontal); 10 s (vertical). (C) Motility fractioned into retrograde, anterograde, and nonmotile events per neuron. Bar graph shows mean ± SEM; two-way ANOVA (ns, P > 0.113) Mock: n = 35 neurons; Hk1 KD: n = 34 neurons. (D) Quantification of flux of BDNF-Qdots in mid-axons. Scatter plot shows mean ± SEM; one-way ANOVA (***, P < 0.0001; ns, P = 0.9994). Mock: n = 54 neurons; Hk1 KD: n = 36 neurons; Hk3 KD: n = 29 neurons. (E) Number of switches in BDNF-Qdot events. Scatter plot shows mean ± SEM; Mann–Whitney t test (**, P = 0.001). Mock: n = 110 events; Hk1 KD: n = 84 events. (F) Pause duration of retrograde BDNF-Qdot events. Scatter plot shows mean ± SEM, Mann-Whitney t test (**, P = 0.0062). Mock: n = 57 events; Hk1 KD: n = 33 events. (G) Net velocity of retrograde BDNF-Qdot events. Scatter plot shows mean ± SEM, unpaired t test (**, P = 0.0061). Mock: n = 63 events; Hk1 KD: n = 34 events.