Figure 4.
Figure 4. G272 defines the phosphoinositide binding selectivity of cytohesin-1. (A) Kd values measured by ITC. NB, no binding. (B) ITC trace of I(1,3,4,5)P4 titrated into diglycine CYTH1 PH domain. (C) ITC trace of diglycine CYTH1 PH domain (aa 243–397) titrated into PI(3,4,5)P3-containing liposomes. (D) Molecular model of the diglycine CYTH1 PH domain bound to I(1,3,4,5)P4 or I(1,4,5)P3. Error estimates shown are from fitting the data using a single site-binding model.

G272 defines the phosphoinositide binding selectivity of cytohesin-1. (A) Kd values measured by ITC. NB, no binding. (B) ITC trace of I(1,3,4,5)P4 titrated into diglycine CYTH1 PH domain. (C) ITC trace of diglycine CYTH1 PH domain (aa 243–397) titrated into PI(3,4,5)P3-containing liposomes. (D) Molecular model of the diglycine CYTH1 PH domain bound to I(1,3,4,5)P4 or I(1,4,5)P3. Error estimates shown are from fitting the data using a single site-binding model.

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