Cytohesin-1 regulates membrane ruffling and actin cytoskeleton rearrangement. (A) Confocal images of cells counterstained with phalloidin (F-actin) and DAPI treated (−, unfilled; +, filled). Arrowheads indicate peripheral membrane ruffles. Scale bar, 20 µm. (B) Kymographs were generated from linescans of the cells’ leading edge imaged between 15 and 60 min after HGF treatment. (C) Quantification of experiments shown in A. (D and E) Quantification of experiments shown in B. All quantified data indicate mean values ± SEM from three or four independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001.