MTCBP-1 resides at invadopodia in pancreatic cancer cells. (A and B) BxPC3 cells stably expressing MTCBP-1-FLAG were seeded on green fluorescent gelatin-coated coverslips, and cells were either stained for FLAG and phalloidin (A) or the invadopodia marker Tks5 (B) to show the localization of MTCBP-1-FLAG at invadopodia. Transverse imaging of invadopodia is shown in B where both Tks5 and MTCBP-1 can be seen at sites of matrix loss. Boxed areas are magnified in insets. Bar, 10 µm.