Rho GTPase pathway perturbed GCs in the context of the morphology landscape. (A–G) “Bag” contours as defined in Fig. 8 D, iii, for control (black) and siRNA (colored) movies. 3–20 GC movies per condition. (H) Separation statistics: control versus KD. Colored bars: 95th percentile bootstrapped CIs. Gray bars: CI between 5th and 95th quartile of the randomized, null distribution. Gray scatter points: same as in Fig. 8, D and E, and Fig. 9. Arrows: Morphology transitions of three example GC trajectories within 90 s after CK666 treatment (25 µM) as identified in Fig. 9 B.