Correlations between GC morphology and motility within an unperturbed, heterogeneous population. (A) Variation in neurite outgrowth rate across 20 control cells (inset, net outgrowth: 10 min. Bar, mean; dark gray regions, 95% CI of the mean; light gray regions, ±SD). (B) Distributions of actin bundle lengths for data in A, sorted by net outgrowth velocity (colors as in A; 3,047–8,213 actin bundles per movie). Boxplots: 25th, 50th, and 75th distribution quantiles. Circles: Mean per movie. Inset: Distribution of means. (C) Spearman correlation matrix of GCA features (left). Schema ball of strongest correlations between net outgrowth rate and GC features (P < 0.05 and false discovery rate (FDR) < 0.2; right). (D) Select morphology features versus net outgrowth rate (as in C). Each data point: single GC movie as in A. Spearman correlation coefficient, r. (E) GCA segmentation overlays (top) and veil-velocity maps (bottom) corresponding to key data points in the net neurite outgrowth rate distribution in A. See Video 4. Bar, 10 µm.