Figure 1.

Automated segmentation of heterogeneous morphologies by GCA. (A) GCA segmentation pipeline: Canonical N1E-115 GC expressing LifeAct GFP. See Video 1. (B) GCA segments GCs of varying veil/stem complexity. See Video 2. (C) Detection of veil-embedded actin bundles. (D) GCA segments GCs of varying filopodia/branch complexity. See Video 3 and Materials and methods. Bar, 10 µm.

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