Src localizes to actin patches in cells with chromatin bridges. (A–E) Localization of Src (A), phosphorylated Src-Y419 (pSrc-Y419; B), phosphorylated FAK-Y925 (pFAK-Y925; C and D), or cortactin (E). Cells were transfected with negative siRNA (control) or siChk1, or treated with PP2 for 5 h. (F) Localization of GFP-Chk1. Broken DNA bridges are indicated by dotted arrows, and the bases of the intercellular canals are indicated by solid arrows. Images are representative of 20 cells from two independent experiments. Insets show 1.6× magnification of the canals bases. Bars, 5 µm.