Complex enrichment analysis reveals the gradual transcriptional down-regulation of v-ATPase in GMCs. (a) The number of significantly differentially regulated complexes between NBs and GMCs increases over time. (b) Venn diagram representation of complexes that are down- or up-regulated in GMCs compared with NBs at a specific time point and their respective overlap. (c) Examples of protein complexes that were progressively down-regulated in GMCs compared with NB at all three time points (v-ATPase, left; Mcm2–7 complex, right). (d) Examples of protein complexes that were progressively up-regulated in GMCs compared with NBs (DRIP–Mediator complex, left; p300–CBP–p270–SWI/SNF complex, right). (e) Examples of protein complexes that were either down- (nuclear origin of replication recognition complex; v-ATPase) or up-regulated (Condensin; NURF complex) between young (1.5 h) and old (5 h) GMCs. (f) Endogenous Vha68-2-GFP protein expression. Vha68-2-GFP shows a strong signal in the cytoplasm and at the membrane of NBs, whereas a reduced signal was detectable in GMCs. Larval brains were stained for F-actin and Mira; arrowheads indicate NBs. (g) Quantification of Vha68-2-GFP protein expression levels in NBs and GMCs. n (NB) = 104, n (GMC) = 205 cells. Error bars represent mean ± SD. ****, P < 0.0001; unpaired two-sided Student’s t test. (h) Schematic of the v-ATPase complex (adapted from Forgac, 2007). A, Vha68-2; H, VhaSFD; a, Vha100-2; e, VhaM9.7-b; d, VhaAC39-1.