Time-resolved transcriptomics demonstrates gradual fate commitment. (a) The number of differentially expressed genes between NBs and GMCs increases over time. (b) Venn diagram representation of genes deregulated between NBs and GMCs at 1.5-, 3-, and 5-h time points and their respective overlap. (c) Unsupervised hierarchical clustering analysis of gene log2fc between NBs and GMCs at the indicated time point with subsequent GO term analysis of each cluster.