Actin role in CME varies with cell type. Clathrin and dynamin lifetimes and productivity of endocytic events in hESCs (A), NPCs (B), and fibroblasts (C) after treatment with DMSO, LatA (1 µM for hESCs and NPCs and 100 nM for fibroblasts), or Jasp (1 µM). Box plots show medians extended from the 25th to 75th percentiles, minimum and maximum data points. ***, P < 0.001, one-way ANOVA and Dunnett’s test versus control (five independent experiments, three to four cells analyzed per experiment, 30–800 structures analyzed).