Endocytic dynamics in three isogenic cell types. (A) TIRFM images of genome-edited, isogenic hESC, NPC, and fibroblast expressing CLTA-TagRFP-T and DNM2-EGFP. Bars, 2 µm. (B) Mean fluorescence intensity profile for CLTA-TagRFP-T (magenta line) and DNM2-EGFP (green line) in the three cell types (three to five independent experiments, three to four cells analyzed per experiment, total numbers of tracks represented: hESCs, n = 240; NPCs, n = 758; fibroblasts, n = 164). (C) Lifetimes of clathrin and dynamin in the indicated cell types. Box plots show median extended from the 25th to 75th percentiles, minimum and maximum data points (three to five independent experiments, three to four cells analyzed per experiment, total numbers of tracks represented: hESCs, n = 240; NPCs, n = 758; fibroblasts, n = 164). ***, P < 0.001, Mann-Whitney test. (D) Immunoblot analysis of cell extracts from the WT and genome-edited (CLTA-TagRFP-T/DNM2-EGFP; CD) hESC line and the NPCs and fibroblasts derived from it.