Search-and-capture expanded by F-actin–driven transport and block of early capture explains chromosome capture dynamics. (A) Renderings of 3D Cytosim simulations of two-staged chromosome congression. Microtubules are in red, chromosomes are blue spheres with green kinetochores. Time starts at NEBD. See also Video 6. (B) Schematics of the different models and corresponding plots of chromosome distance to the AP over time and capture events. Trajectories are color-coded for actin- (green) and microtubule (red)-driven transport phases and arrival at the spindle (gray). Chromosome capture events are represented by gray dots. (C) Comparison of simulated and experimental chromosome capture dynamics (observed in oocytes, treated with Latrunculin B or DMSO; Fig. 2). Data from 13 oocytes are shown for both simulations and experiments. (D) Plots of individual experimental and simulated capture events and histograms of the same data. S&C, search and capture; trans., transport.