The F-actin network does not prevent chromosome capture and transport by microtubules, but transport along microtubules interferes with F-actin network integrity. (A) A single selected slice from a deconvolved confocal stack of an oocyte fixed 5 min after NEBD and stained for tubulin (red), F-actin (green), and chromosomes by using Draq5 (cyan). (B) Microtubule length distribution measured in control and Latrunculin B–treated oocytes and plotted against each other. (C) Selected single confocal sections acquired over time showing the nuclear area of an oocyte expressing 3mEGFP-UtrCH (gray) and H2B-mCherry (cyan). See also Video 4. Right: Zoom-in on the region marked by a dashed rectangle of a chromosome transported along a microtubule (red arrowhead) causing local collapse of the F-actin network. (D) Maximum-intensity z-projections of a 3D confocal time series through the nuclear region of an oocyte expressing H2B-mCherry (cyan) and injected with UtrCH–Alexa Fluor 568 (gray). Single z-slice zooms of the regions marked by dashed rectangles are shown below visualizing the disruption of the F-actin network where chromosomes are pulled through. Bars: (main images) 10 µm; (C, inset) 5 µm. Time is given as minutes:seconds relative to NEBD for all panels.